Čes. stomatol. Prakt. zub. lék. (Czech Dental Journal) 2008; 108(5): 122-128

Dental Pulp - the Source of Mesenchymal Stem Cells

J. Suchánek1,*, T. Soukup2, R. Ivančaková1, B. Víšek2
1 Stomatologická klinika LF UK a FN, Hradec Králové
2 Ústav histologie a embryologie LF UK, Hradec Králové

Objective: the study aimed to isolation of dental pulp stem cells (DPSC), determination of proliferative and differentiation potential and observation of morphological changes and viability of DPSC cultured in vitro.

Methods: the authors used a total of 16 DPSC cell lines isolated from the third molars. The cells were cultured in a modified medium for mesenchymal stem cells (MSC) enriched with growth factors (EGF, PDGF), 2% FCS and ITS supplement. For the determination of quantitative parameters and cell viability the Vi-Cell XR and Z2 Counter were used.

Results: Enzyme dissociation of the complete dental pulp provided isolation of 45 ± 6 (10- 108) stem cells. These cells were cultured through 60 population duplications (PD) in a modified medium for MSC. The analysis revealed increased time required for duplication from original 15-23 h for the first PD to 24-35 h for the subsequent 30 PD. Viability of the cultured cells was continually 96 ± 3%. In the course of the long-term cultivation of DPSC no signs of culture degeneration or spontaneous differentiation of the cells were observed.

Conclusion: these cells represent a morphologically homogenous population with a marked proliferation potential and stable phenotype in cultivation conditions in vitro. The dental pulp stem cells represent a highly potent source of histocompatible stem cells for cellular therapy, which is not limited to diseases of dental pulp and periodontium only. Their broad differentiation potential opens the way for application of these cells in other medical branches.

Keywords: stem cells; dental pulp stem cells; dental pulp; cultivation

Published: December 1, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Suchánek J, Soukup T, Ivančaková R, Víšek B. Dental Pulp - the Source of Mesenchymal Stem Cells. Čes. stomatol. Prakt. zub. lék. 2008;108(5):122-128.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anker, P. S., Scherjon, S. A., Kleijburg-van der Keur, C. et al.: Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood, roč. 102, 2003, s. 1548-1549. Go to original source... Go to PubMed...
    2. Bruder, S. P., Kraus, K. H., Goldberg, V. M., Kadiyla, S.: The effect of implants loaded with autologous mesenchymal stem cells on thehealing of canine segmental bone defects. J. Bone Joint Surg. Am., roč. 80, 1998, s. 985-996. Go to original source... Go to PubMed...
    3. Campagnoli, C., Roberts, I. A., Kumar, S. et al.: Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood, roč. 98, 2001, s. 2396-2402. Go to original source...
    4. Colter, D. C., Class, R., DiGirolamo, C. M. et al.: Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Proc. Natl. Acad. Sci. USA, 2000, č. 97, s. 3213-3218. Go to original source... Go to PubMed...
    5. Doetsch, F.: A niche for adult neural stem cells. Curr. Opin. Genet. Dev., 2003, č. 13, s. 543-550. Go to original source... Go to PubMed...
    6. Gronthos, S., Brahim, J., Li, W. et al.: Stem cell properties of human dental pulp stem cells. J. Dent. Res., roč. 81, 2002, č. 8, s. 531-535. Go to original source... Go to PubMed...
    7. Gronthos, S., Mankani, M., Brahim, J. et al.: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc. Natl. Acad. Sci. USA, 2000, č. 97, s. 13625-13630. Go to original source... Go to PubMed...
    8. Jendelová, P., Herynek, V., De Cross, J., Glogárová, K., Andersson, B., Hájek, M., Syková, E.: Imaging the fate of implanted bone marrow stromal cells labeled with superparamagnetic nanoparticles. Magn. Reson. Med., roč. 50, 2003, s. 767-776. Go to original source... Go to PubMed...
    9. Jiang, Y., Jahagirdar, B. N., Reinhardt, L. et al.: Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, roč. 418, 2002, s. 41-49, supplementary information www.nature.com/nature. Go to original source...
    10. Liu, H., Gronthos, S., Shi, S.: Dental pulp stem cells. Methods Enzymol., 419, 2006, s. 99-113. Go to original source... Go to PubMed...
    11. Makino, S., Fukuda, K., Miyoshi, S., Konishi, F., Kodama, H., Pan, J., Sano, M., Takahashi, T., Hoti, S., Abe, H., Hata, J., Umezawa, A., Ogawa, S. Cardiomyocytes can be generated from marrow stromal cells in vitro. J. Clin. Invest., roč. 103, 1999, s. 697-705. Go to original source... Go to PubMed...
    12. Minguel, J. J., Erices, A., Conget, P.: Mesenchymal stem cells. Exp Biol Med, 2001, č. 226, s. 507-520. Go to original source... Go to PubMed...
    13. Miura, M., Gronthos, S., Zhao, M., Lu, B., Fischer, L. W., Robey, P. G., Shi, S.: SHED: Stem cells from human exfoliated deciduous teeth. Proc. Natl. Acad. Sci. USA, 100, 2003, s. 5807-5812. 80. Mesenchymal stem cells. Exp. Biol. Med., 226, 2001, s. 507-520. Go to original source... Go to PubMed...
    14. Nakahara, H., Dennis, J. E., Bruder, S. P. et al.: In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells. Exp. Cell. Res., roč. 195, 1991, s. 492-503. Go to original source... Go to PubMed...
    15. Pereira, R. F., Halford, K. W., O'Hara, M. D. et al.: Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage and lung in irradiated mice. Proc. Natl. Acad. Sci. USA, 1995, č. 92, s. 4857-4861. Go to original source... Go to PubMed...
    16. Perry, B. C., Zhou, D., Wu, X., Yang, F. C., Byers, M. A., Chu, T. M., Hockema, J. J., Woods, E. J., Goebel, W. S: Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use tissue. Eng. Part C Methods, 14,2008, 2, s. 149-156. Go to original source... Go to PubMed...
    17. Pittenger, M. F., Mackay, A. M., Beck, S. C. et al.: Multilineage potential of adult human mesenchymal stem cells. Science, 1999, č. 284, s. 143-147. Go to original source... Go to PubMed...
    18. Pittenger, M. F., Martin, B. J.: Mesenchymal stem cells and their potential as cardiac therapeutics. Circ. Res., roč. 95, 2004, s. 9-20. Go to original source... Go to PubMed...
    19. Reyes, M., Lund, T., Lenvik, T. et al.: Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood, roč. 98, 2001, č. 9, s. 2615-2625. Go to original source... Go to PubMed...
    20. Sanchez-Ramos, J. et al.: Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp. Neurol., 2000, č. 164, s. 247-256. Go to original source... Go to PubMed...
    21. Seo, B. M., Miura, M., Gronthos, S., Bartold, P. M., Batouli, S. et al.: Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet, roč. 364, 2004, s. 149-155. Go to original source... Go to PubMed...
    22. Shi, S., Gronthos, S.: Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp. J. Bone Miner. Res., roč. 18, 2003, č. 4, s. 696. Go to original source... Go to PubMed...
    23. Soukup, T., Mokrý, J., Karbanová, J., Pytlík, R., Suchomel, P., Kučerová, L.: Mesenchymal stem cells isolatér from human bone marrow: cultivation, phenotypic analysis and changes in proliferation kinetice. Acta Medica, Hradec Králové, roč. 49, 2006, 1, s. 27-33. Go to original source... Go to PubMed...
    24. Suchánek, J., Soukup, T., Ivančaková, R., Karbanová, J., Hubková, V., Pytlík R., Kučerová, L.: Human dental pulp stem cells-isolation and long term cultivation. Acta Medica, Hradec Králové, roč. 50, 2007, s. 195-201. Go to original source... Go to PubMed...
    25. Werntz, J. R., Lane, J. M., Burstein, A. H., Justin, R., Klein, R., Tomin, E.: Qualitative and quantitative analysis of orthotopic bone regeneration by marrow. J. Orthop. Res., roč. 14, 1996, s. 85-93. Go to original source... Go to PubMed...
    26. Zuk, P. A., Zhu, M., Aashijian, P., et al.: Human adipose tissue is a source of multipotent stem cells. Mol. Biol. Cell., roč. 13, 2002, s. 4279-4295. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content