Czech Dental Journal, 2016 (vol. 116), issue 1

Original article

In Vitro Cultivation of Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth in Low-Xenogeneic-Serum Containing Media

T. Suchánková Kleplová, K. Z. Browne, T. Soukup, J. Suchánek

Čes. stomatol. Prakt. zub. lék. (Czech Dental Journal) 2016; 116(1): 3-11 | DOI: 10.51479/cspzl.2016.001

Introduction and aims: Recently, the human regenerative medicine has been departing from the use of xenogeneic materials for the possibility of zoonosis transfer and development of prion infection. We have focused our research on cultivation of Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth (SHED) in a medium with lowered concentration of Fetal Calf Serum (FCS), which decreases the chances of the above mentioned negative impacts on the cultivated cell population. In 2011, Karbanova et al. have shown that the Dental Pulp Stem Cells (DPSCs) from Impacted Third Molars can be viably cultured in a xenogeneic medium containing as little...

Oral Carriage of Staphylococcus aureus among Dental Patients in Dependence on Conditions in Oral Cavity

A. Kaiglová, J. Špajdelová, M. Stanko, E. Dubajová

Čes. stomatol. Prakt. zub. lék. (Czech Dental Journal) 2016; 116(1): 12-19 | DOI: 10.51479/cspzl.2016.002

Introduction and aim: Staphylococcus aureus is a significant opportunistic pathogen possessing highly variable genome, causing thus wide range of infections. An important location where this pathogen may occur in healthy individuals includes not only organs such as skin, pharynx, nasal mucosa, etc., but also oral cavity. The main objective of our study was to find out whether various oral cavity conditions such as periodontitis, fixed and removable dental prostheses and stomatological diseases with indications for extraction influence the level of oral cavity colonization by the Staphylococcus aureus, as well as to define the frequency...

Review

Maturogenesis. Part I. Introduction, Stem Cells, Growth Factors and Matrix

R. Žižka, J. Šedý, J. Škrdlant, N. Němcová

Čes. stomatol. Prakt. zub. lék. (Czech Dental Journal) 2016; 116(1): 20-26 | DOI: 10.51479/cspzl.2016.003

Objectives: Endodontic treatment of immature permanent tooth with necrotic pulp is one of the most challenging treatment options in endodontics. Even when the standard treatment modalities like calcium hydroxide apexification or MTA plug are succesfull, the long term prognosis of teeth is rather to be poor. It is because of thin root canal walls, which are prone to fracture. The great progress has been achieved in last two decades in the field of tissue engineering which leads to novel treatment strategies. Its aim is formation at new vital tissue inside of root canal system. This new tissue should be able to produce hard tissue, that leads...